Methods for prevention of mycoplasma contamination in cell culture 1. Improve aseptic techniques and practices. 2. Test cultures for contamination. 3. Use antibiotics responsibly. 4. Discard or treat mycoplasma contaminated cells. 5. Quarantine new cells of any origin. 6. Reduce aerosol generation.

The easiest way to eliminate mycoplasma is to autoclave the contaminated cells together with any bottle of medium and solution used with this relevant culture. Don't forget subsequent cleaning and disinfection of surfaces, hoods, incubators, pipettors etc.