Next generation sequencing (NGS) refers to large-scale DNA sequencing technology that allows for querying the entire genome (whole genome), the exons within all known genes (whole exome), or only exons of selected genes (target panel). From: Accurate Results in the Clinical Laboratory (Second Edition), 2019.

Next generation sequencing (NGS) refers to a versatile technology of DNA sequencing that permits high-throughput, highly adaptable whole genome-scale assays at reasonable cost and high accuracy.

NGS revolutionizes genomic research by setting a new standard. NGS has a tremendous potential for new discovery in multiple biological science disciplines. This technology enables massively parallel sequencing of nucleic acid fragments to address a variety of experimental questions. The ultra-high-throughput and accuracy of NGS opens countless possibilities of genome-scale assays in clinical, medical, cancer and basic research, screening large numbers of samples for changes underpinning diseases and traits of interest. New systems and platforms, fast evolving methodologies, bioinformatics and innovative application of NGS in combination with other technologies change the landscape of what is possible today.

Next-Generation Sequencing is a process of sequencing multiple fragments of DNA or RNA in a parallel way with speed and accuracy. It deeply and rapidly sequences a whole-genome and target sequences, helpful in studying cancer samples, etc.

The principle of Next Generation Sequencing (NGS) is similar to that of Sanger sequencing,in which genomic strand is fragmented, and the bases in each fragment are identified by emitted signals when the fragments are ligated against a template strand.